■ 基本信息
別名: | pHT-304 |
啟動子: | Pgrac |
復(fù)制子: | pUC |
質(zhì)粒分類: | 廣宿主系列，枯草桿菌載體 |
質(zhì)粒大小: | 6530bp |
質(zhì)粒標(biāo)簽: | LacZa |
原核抗性: | Amp |
真核抗性: | Erm |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 枯草芽孢桿菌 |
誘導(dǎo)方式: | IPTG誘導(dǎo) |
5'測序引物: | M13R:CAGGAAACAGCTATGACC |
3'測序引物: | M13F:TGTAAAACGACGGCCAGT |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 枯草桿菌 |
質(zhì)粒用途: | 蛋白表達 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡介
The promoter of pHT304 vector is Lac, which can be used to induce the expression of recombinant protein in Escherichia coli DE3 engineering bacteria (DE3 BL21 or DE3 Rosetta). The tag has LacZa, which can be purified by nickel column.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1012pHT304枯草胞內(nèi)質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。
