■ 基本信息
啟動子: | ADH1 |
復制子: | 2μ |
質粒分類: | 酵母系列，酵母雙雜交載體 |
質粒大小: | 10.kb |
原核抗性: | Amp |
真核抗性: | LEU2 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 酵母細胞 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物: | 根據(jù)序列設計引物 |
■ 質粒屬性
質粒宿主: | 酵母菌 |
質粒用途: | 雜交 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | LEU2
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熒光標記: |
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■ 質粒簡介
pGADT7-T encodes a fusion of the SV40 large T-antigen (a.a. 86–708) and the GAL4 AD (a.a. 768–881). The SV40 large T DNA (GenBank Locus SV4CG) was derived from a plasmid referenced in Li & Fields (1993) and was cloned into pGADT7 using the EcoR I and Xho I sites. pGADT7-T has not been sequenced.
■ 質粒圖譜
■ 質粒序列
質粒序列可參考（具體以測序為準）下載：
ZK976 pGADT7-T酵母雙雜交質粒.txt
