■ 基本信息
別名: | pGADT7-AD |
啟動(dòng)子: | ADH1 |
復(fù)制子: | 2μ |
質(zhì)粒分類: | 酵母系列，酵母雙雜交載體 |
質(zhì)粒大小: | 7987bp |
原核抗性: | Amp |
真核抗性: | LEU2 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 酵母細(xì)胞 |
5'測(cè)序引物: | T7:TAATACGACTCACTATAGGG |
3'測(cè)序引物:
| 根據(jù)序列設(shè)計(jì)引物
|
備注: | 酵母雙雜交空載體 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 雜交 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | LEU2
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡(jiǎn)介
pGADT7 AD is a yeast expression vector that is designed to express a protein of interestfused to a GAL4 activation domain (AD; amino acids 768–881). Transcription of the GAL4 ADfusion is driven by the constitutively active ADH1 promoter (PADH1), and is terminated at theADH1 transcription termination signal (TADH1). The GAL4 AD fusion contains an N-terminalSV40 nuclear localization signal (SV40 NLS; 1) that targets the protein to the yeast nucleus,and a hemagglutinin epitope tag (HA Tag), located between the GAL4 AD and the proteinof interest, that allows the protein to be easily detected with HA-tag antibodies.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK974pGADT7（別名pGADT7-AD）酵母雙雜交質(zhì)粒.txt
