■ 基本信息
啟動子: | TRP1，T3,T7 |
復制子: | pUC |
質粒分類: | 酵母系列，畢赤酵母表達載體 |
質粒大小: | 4783bp |
原核抗性: | Amp |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 酵母細胞 |
誘導方式: | 半乳糖誘導 |
5'測序引物: | M13R:CAGGAAACAGCTATGACC |
3'測序引物: | 根據序列設計引物 |
■ 質粒屬性
質粒宿主: | 酵母菌 |
質粒用途: | 蛋白表達 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | TRP1
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熒光標記: |
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■ 質粒簡介
YCtype centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ) peptide. pRSS56, constructed by ligating a PvuI fragment (bp 4982412) of pBluescript KS+ to a PvuI fragment (bp 2850 730) of pBS(+), contains the KS MCS from pBluescript KS(+) and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A genomic HindII/PstI fragment (1.002 kb) containing the TRP1 gene was inserted into the NdeI site and a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) was inserted into the AatII site of pRSS56. All ends were blunted. An EcoRI site in the TRP1containing fragment (external to the coding sequence) was destroyed. The order of the major features in this plasmid is: TRP1f1 ori (NaeI) T7 promoter lacZ/MCS T3 promoter pMB1 ori bla CEN6 ARSH4.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK956pRS314釀酒酵母質粒.txt
