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莊盟生物

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產(chǎn)品名稱:ptdTomato-N1質(zhì)粒

貨號(hào) 規(guī)格 價(jià)格 訂購數(shù)量 是否現(xiàn)貨
ZK436 1μg(20μl,50ng/μl) 870 - + 有貨

基本信息

啟動(dòng)子:

CMV

復(fù)制子:

pUC

終止子:

SV40 poly(A) signal

質(zhì)粒分類:

哺乳細(xì)胞,熒光蛋白報(bào)告載體

質(zhì)粒大小:

5443bp

原核抗性:

Kan

真核抗性:

G418

克隆菌株:

DH5a

培養(yǎng)條件:

37度

表達(dá)宿主:

哺乳細(xì)胞

誘導(dǎo)方式:

無須誘導(dǎo),瞬時(shí)表達(dá)

5'測(cè)序引物:
CMV-F:CGCAAATGGGCGGTAGGCGTG

3'測(cè)序引物:

根據(jù)序列設(shè)計(jì)引物


質(zhì)粒屬性

質(zhì)粒宿主:

哺乳細(xì)胞

質(zhì)粒用途:

蛋白表達(dá)

片段類型:

ORF

片段物種:


原核抗性:

Kan

真核抗性:

G418

熒光標(biāo)記:



質(zhì)粒簡(jiǎn)介

ptdTomato-N1 is a mammalian expression vector designed to express a protein of interest fused to the N-terminus of tdTomato. td Tomato is a member of the family of fruit fluorescent proteins (1) derived from the Discosoma sp. red fluorescent protein, DsRed (2). Because the Tomato protein has a tendency to dimerize, the vector was designed with two copies of the Tomato coding region linked together to allow intramolecular dimerization. As a result, each tdTomato RNA transcript encodes a tandem dimer of the Tomato protein (excitation and emission maxima equal 554nm and 581nm, respectively). Expression of td Tomato as a tandem dimer prevents the fused protein of interest from being forced into a dimeric complex. Fusions that retain the fluorescence properties of tdTomato can be monitored by flow cytometry and localized by fluorescence microscopy. The multiple cloning site (MCS) in ptdTomato-N1 is positioned upstream of the tdTomato coding sequence. A Kozak consensus sequence, located between the MCS and the tdTomato coding sequence, enhances translational efficiency of the unfused tdTomato protein in eukaryotic cells (3). SV40 polyadenylation signals downstream of the tdTomato coding sequence direct proper processing of the 3' end of the tdTomato (or fusion gene) mRNA.

質(zhì)粒只保證關(guān)鍵序列正確,不保證表達(dá)效果。

質(zhì)粒圖譜



質(zhì)粒序列

質(zhì)粒序列請(qǐng)下載:ZK436ptdTomato-N1哺乳熒光質(zhì)粒.txt

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總價(jià)格:¥2000
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