■ 基本信息
別名: | pETBlue2 |
啟動(dòng)子: | T7 |
復(fù)制子: | pBR322 |
終止子: | T7 terminator |
質(zhì)粒分類(lèi): | 大腸桿菌載體；PET系列表達(dá)質(zhì)粒 |
質(zhì)粒大小: | 3653bp |
質(zhì)粒標(biāo)簽: | C-HSV，C-6×His |
原核抗性: | Amp |
真核抗性: | LacZ |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，有氧，LB |
表達(dá)宿主: | 大腸桿菌BL21(DE3)pLacI |
誘導(dǎo)方式: | IPTG或乳糖及其類(lèi)似物 |
5'測(cè)序引物:
| T7:TAATACGACTCACTATAGGG
|
3'測(cè)序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類(lèi)型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡(jiǎn)介
pETBlue-2是一個(gè)大腸桿菌pET系列表達(dá)質(zhì)粒。The pETBlue?-2 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-2 defines the open reading frame and inserts must be cloned in-frame if expression is desired. The vector features an expanded multiple cloning site (MCS) and optional C-terminal HSV?Tag? and His?Tag? sequences. The sequence is numbered from the first base of the T7 promoter sequence.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK196pETBlue-2大腸表達(dá)質(zhì)粒.txt
